Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes

doi: 10.1073/pnas.1519395113

Figure Lengend Snippet: Factors screened by tubule culture experiments for their roles in the proliferation and differentiation of stem Leydig cells

Article Snippet: This same sequence occurs in vivo ( 21 , 22 ). table ft1 table-wrap mode="anchored" t5 Table S1. caption a7 Reagent Signaling pathway Concentration (range tested) Manufacturer Ovine LH 10 ng/mL (0.1–10) Hormone Program USDA Rat FSH 20 ng/mL (0.2–20) Hormone Program NIDDK T3 10 nM (0.1–10) Sigma-Aldrich Estradiol (E2) 10 ng/mL (0.1–10) Steraloids DHT 10 ng/mL (0.1–10) Steraloids R1881 AR − 10 nM (0.1–10) Sigma-Aldrich Dexamethasone 10 nM (0.1–10) Sigma-Aldrich Retinoic acid 1 μM (0.01–1) Sigma-Aldrich Mouse PDGFAA 10 ng/mL (0.1–10) Prospec Protein Specialists Rat PDGFBB 10 ng/mL (0.1–10) Sigma-Aldrich PDGFR Inhibitor IV PDGFBB − 20 nM (0.2–20) EMD Bioscience Imatinib Mesylate c-Kit − /PDGFR − 0.2 μM (0.02–2) Selleck Chemicals Human TGFβ1 10 ng/mL (0.1–10) Cell Signaling Technology Human Activin 10 ng/mL (0.1–10) Sigma-Aldrich SB431542 TGFβ − /Activin − 0.1 μM (0.01–1) Selleck Chemicals SB525334 TGF-β − 50 nM (0.5–50) Selleck Chemicals Rat TGFα 10 ng/mL (0.1–10) Sigma-Aldrich Mouse EGF 10 ng/mL (0.1–10) Sigma-Aldrich Porcine Inhibin 10 ng/mL (0.1–10) Sigma-Aldrich Rat IL-1β 1 ng/mL (0.1–10) Sigma-Aldrich Rat IGF1 50 ng/mL (0.5–50) Sigma-Aldrich Human SCF 10 ng/mL (0.1–10) MP Biomedicals Rat GDNF 10 ng/mL (0.1–10) EMD Chemicals Human FGF2 10 ng/mL (0.1–10) Novus Biologiclas Mouse LIF 10 ng/mL (0.1–10) Sigma-Aldrich DAPT Notch − 2 μM (0.2–20) EMD Bioscience Lithium ?

Techniques: